McArdle Laboratory, 421A
1400 University Avenue
Madison, WI 53706
Lab Phone: (608) 262-9834
Publications
Recent Publications
  • Powell, E., Shanle, E., Brinkman, A., Li, J., Keles, S., Wisinski, K. B., Huang, W., and Xu, W.  Identification of Estrogen Receptor Dimer Selective Ligands Reveals Growth-Inhibitory Effects on Cells That Co-Express ERa and ERβ.  PLoS ONE, 7(2):e30993, 2012.
  • Wen, Z., Pyeon, D., Wang, Y., Lambert, P., Xu, W., and Ahlquist, P.  Orphan Nuclear Receptor PNR/NR2E3 Stimulates p53 Functions by Enhancing p53 Acetylation.  Mol. Cell. Biol., 32:  26-35, 2012.
  • Wu, J., and Xu, W.  Histone H3R17me2a Mark Recruits Human RNA Polymerase-Associated Factor 1 Complex to Activate Transcription.  Proc. Natl. Acad. Sci. USA, in press, 2012 [Epub ahead of print Mar 26 2012].
  • Al-Dhaheri, M., Wu, J., Skliris, G. P., Li, J., Higashimato, K., Wang, Y., White, K. P., Lambert, P., Zhu, Y., Murphy, L., and Xu, W.  CARM1 Is an Important Determinant of ERα-Dependent Breast Cancer Cell Differentiation and Proliferation in Breast Cancer Cells.  Cancer Res., 71: 2118-2128, 2011. [Epub ahead of print Jan 31 2011].
  • Chumanov, R. S., Kuhn, P. A., Xu, W., and Burgess, R. R.  Expression and Purification of Full-Length Mouse CARM1 from Transiently Transfected HEK293T Cells Using HaloTag Technology.  Protein Expr. Purif., 76: 145-153, 2011.
  • Kuhn, P., Chumanov, R., Wang, Y., Ge, Y., Burgess, R. R., and Xu, W.  Automethylation of CARM1 Allows Coupling of Transcription and mRNA Splicing.  Nucleic Acids Res., in press, 2011 [Epub ahead of print Dec 7 2010].
  • Shanle, E. K., and Xu, W.  Endocrine Disrupting Chemicals Targeting Estrogen Receptor Signaling:  Identification and Mechanisms of Action.  Chem. Res. Toxicol., 24:  6-19, 2011.
  • Huang, S.-X., Powell, E., Rajski, S. R., Zhao, L.-X., Jiang, C.-L., Duan, Y., Xu, W., and Shen, B.  Discovery and Total Synthesis of a New Estrogen Receptor Heterodimerizing Actinopolymorphol A from Actinopolymorpha rutilus.  Org. Lett., 12: 3525-3527, 2010.
  • Shanle, E. K., and Xu, W.  Selectively Targeting Estrogen Receptors for Cancer Treatment.  Adv. Drug Deliv. Rev., 62: 1265-1276, 2010. 
  • Yang, Y., Lu, Y., Espejo, A., Wu, J., Xu, W., Liang, S., and Bedford, M. T.  TDRD3 Is an Effector Molecule for Arginine-Methylated Histone Marks.  Mol. Cell, 40:  1016-1023, 2010.
  • Charoensuksai, P., and Xu, W.  PPARs in Rhythmic Metabolic Regulation and Implications in Health and Disease.  PPAR Res., 2010:243643, 2010.
  • Huang, S.-X., Powell, E., Rajski, S. R., Zhao, L.-X., Jiang, C.-L., Duan, Y., Xu, W., and Shen, B.  Discovery and Total Synthesis of a New Estrogen Receptor Heterodimerizing Actinopolymorphol A from Actinopolymorpha rutilus.  Org. Lett., 12: 3525-3527, 2010.
  • Powell, E., Huang, S.-X., Xu, Y., Rajski, S. R., Wang, Y., Peters, N., Guo, S., Xu, H. E., Hoffmann, F. M., Shen, B., and Xu, W.  Identification and Characterization of a Novel Estrogenic Ligand Actinopolymorphol A.  Biochem. Pharmacol., 80: 1221-1229, 2010.
  • Powell, E., Wang, Y., Shapiro, D. J., and Xu, W.  Differential Requirements of Hsp90 and DNA for the Formation of Estrogen Receptor Homodimers and Heterodimers.  J. Biol. Chem., 285: 16125-16134, 2010.
  • Powell, E., Xie, W., and Xu, W.  Molecular Players and Cellular Pathways in Estrogen-Modulated Breast Cancer.  In:  Female Sex Hormones and Cancers, Chapter 2, pp. 25-62.  New York:  Nova Science Publishers, Inc., 2010.
  • Powell E., Wang Y., Shapiro D.J., Xu, W. (2010) Differential requirements of Hsp90 and DNA for the formation of estrogen receptor homodimers and heterodimers. J. Biological Chemistry, 285: 16125-16134.
  • Kuhn, P., Xu, W. Nuclear receptor coregulators and beyond. (2009) Progress in Molecular Biology and Translational Science, Volume 87, 297-340.
  • Kuhn, P., Xu, Q., Cline, E., Zhang, D., Ge, Y. and Xu, W. (2009) Delineating Anapheles Gambiae Coactivator Associated Arginine Methyltransferase 1 (AgCARM1) Automethylation Using Top-Down High Resolution Tandem Mass Spectrometry. Protein Science, Volume 18:1272-1280.
  • Nofsinger, R. R., Li, P., Hong, S.-H., Jonker, J. W., Barish, G. D., Ying, H., Cheng, S.-y., LeBlanc, M., Xu, W., Pei, L., Kang, Y.-J., Nelson, M., Downes, M., Yu, R. T., Olefsky, J. M., Lee, C.-H., and Evans, R. M. SMRT Repression of Nuclear Receptors Controls the Adipogenic Set Point and Metabolic Homeostasis. Proc. Natl. Acad. Sci. USA, 105: 20021-20026, 2008.
  • Powell, E., and Xu, W. Intermolecular Interactions Identify Ligand-selective Activity of Estrogen Receptor a/b Dimers. Proc. Natl. Acad. Sci. USA, 105: 19012-19017, 2008.
  • Zhu, Y., Zhu, Y., and Xu, W.  EzArray:  A Web-based Highly Automated Affymetrix Expression Array Data Management and Analysis System.  BMC Bioinformatics, 9:46, 2008.
  • Higashimoto K., Kuhn, P., Desai, D., Cheng, X., Xu, W. Phosphorylation-mediated inactivation of coactivator-associated arginine methyltransferase 1. Proc. Natl. Acad. Sci., U.S.A., 104(30): 12318-12323, 2007.
  • Powell, E., Kuhn, P. and Xu, W. Nuclear Receptor Cofactors in PPARg-Mediated  Adipogenesis and Adipocyte Energy Metabolism (2007) PPAR Res, 2007: 53843.
 
 
Previous Publications
  • Yao, T, Song, L, Xu, W., DeMartino G. N., Florens, L., Swanson, S.K., Washburn, M.P., Conaway, R.C., Conaway, J.W., and Cohen, R.E. Proteasome recruitment and activation of the Uch37 deubiquitinating enzyme by Adrm1. Nature Cell Biology, 8 (9): 994-1002, 2006.
  • Xu, W. Nuclear receptor coactivators: the key to unlock chromatin. Biochem. Cell Biol. 83: 1-11, 2005
  • Xu, W., Cho, H., Kadam, S., Banayo, E. M., Anderson, S., Yates III, J. R., Emerson, B. M., and Evans, R. M. A Methylation-Mediator Complex in Hormone Signaling. Genes Dev., 18: 144-156, 2004.
  • Louie, M. C., Yang, H. Q., Ma, A.-H., Xu, W., Zou, J. X., Kung, H.-J., and Chen, H.-W. Androgen-induced Recruitment of RNA Polymerase II to a Nuclear Receptor-p160 Coactivator Complex. Proc. Natl. Acad. Sci., U.S.A., 100(5): 2226-2230, 2003.
  • Xu, W., Cho, H., and Evans, R. M. Acetylation and Methylation in Nuclear Receptor Gene Activation. Methods Enzymol., 364: 205-223, 2003.
  • Demarest, S. J., Martinez-Yamout, M., Chung, J., Chen, H., Xu, W., Dyson, H. J., Evans, R. M., and Wright, P. E. Mutual Synergistic Folding in Recruitment of CBP/p300 by p160 Nuclear Receptor Coactivators. Nature, 415: 549-553, 2002.
  • Xu, W., Chen, H., Du, K., Asahara, H., Tini, M., Emerson, B. M., Montminy, M., and Evans, R. M. A Transcriptional Switch Mediated by Co-Factor Methylation. Science, 294: 2507-2511, 2001.
  • Lam, Y.A., Xu, W., DeMartino, G., and Cohen, R. E. Editing of ubiquitin conjugates by an isopeptidase in the 26S proteasome, Nature 385: 737-740, 1997.